Journal: Cell reports

Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

doi: 10.1016/j.celrep.2023.113282

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-MiTF antibody , Abcam , Cat# ab122982; RRID:AB_10902226.

Techniques: Recombinant, Plasmid Preparation, Software

Journal: iScience

Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

doi: 10.1016/j.isci.2022.105238

Figure Lengend Snippet: Mean number of cells per millimeter for each marker

Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

Techniques:

Journal: iScience

Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

doi: 10.1016/j.isci.2022.105238

Figure Lengend Snippet: A subpopulation of immature melanocytes exists in the interfollicular epidermis that differentiates temporarily during radiation exposure Mean number of cells per millimeter in the basal layer (n = 15 patients). (A) MITF-positive melanocytes ( ) and Bcl-2-positive melanocytes ( ). (B) MITF-negative cells ( ) and Bcl-2-negative cells ( ) are morphologically characterized as melanocytes. Error bars represent SEM.

Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

Techniques:

Journal: iScience

Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

doi: 10.1016/j.isci.2022.105238

Figure Lengend Snippet: Number of negative epidermal melanocytes per millimeter and percentage of the total number melanocytes counted for each marker

Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

Techniques: Control

Journal: iScience

Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

doi: 10.1016/j.isci.2022.105238

Figure Lengend Snippet:

Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

Techniques: Recombinant, Plasmid Preparation, Software

Journal: Biomedicines

Article Title: RICTOR Affects Melanoma Tumorigenesis and Its Resistance to Targeted Therapy

doi: 10.3390/biomedicines9101498

Figure Lengend Snippet: RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).

Article Snippet: Membranes were probed with the following primary antibodies: Myc-Tag, RICTOR, ACTIN, p-AKT (S473), AKT, p-ERK, ERK, pSTAT3 (Y705), STAT3, SOX10, AXL (Cell Signaling Technology, Danvers, MA, USA), and MITF (OriGene Technologies Inc, Rockville, MD, USA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Expressing